In vitro anti-hepatitis B and SARS virus activities of a titanium-substituted-heteropolytungstate.
Identifieur interne : 001D04 ( Main/Exploration ); précédent : 001D03; suivant : 001D05In vitro anti-hepatitis B and SARS virus activities of a titanium-substituted-heteropolytungstate.
Auteurs : Yan-Fei Qi [République populaire de Chine] ; Hong Zhang ; Juan Wang ; Yanfang Jiang ; Jinhua Li ; Ye Yuan ; Shiyao Zhang ; Kun Xu ; Yangguang Li ; Juan Li ; Junqi Niu ; Enbo WangSource :
- Antiviral research [ 1872-9096 ] ; 2012.
Descripteurs français
- KwdFr :
- ADN viral (), Animaux, Antigènes de la nucléocapside du virus de l'hépatite virale B (analyse), Antigènes de surface du virus de l'hépatite B (analyse), Antigènes e du virus de l'hépatite virale B (analyse), Antiviraux (), Antiviraux (pharmacologie), Antiviraux (synthèse chimique), Cellules HepG2, Cellules Vero, Composés du tungstène (), Composés du tungstène (pharmacologie), Effet cytopathogène viral (), Humains, Réplication virale (), Tests de sensibilité microbienne, Virus de l'hépatite B (), Virus du SRAS ().
- MESH :
- analyse : Antigènes de la nucléocapside du virus de l'hépatite virale B, Antigènes de surface du virus de l'hépatite B, Antigènes e du virus de l'hépatite virale B.
- pharmacologie : Antiviraux, Composés du tungstène.
- synthèse chimique : Antiviraux.
- ADN viral, Animaux, Antiviraux, Cellules HepG2, Cellules Vero, Composés du tungstène, Effet cytopathogène viral, Humains, Réplication virale, Tests de sensibilité microbienne, Virus de l'hépatite B, Virus du SRAS.
English descriptors
- KwdEn :
- Animals, Antiviral Agents (chemical synthesis), Antiviral Agents (chemistry), Antiviral Agents (pharmacology), Chlorocebus aethiops, Cytopathogenic Effect, Viral (drug effects), DNA, Viral (drug effects), Hep G2 Cells, Hepatitis B Core Antigens (analysis), Hepatitis B Surface Antigens (analysis), Hepatitis B e Antigens (analysis), Hepatitis B virus (drug effects), Humans, Microbial Sensitivity Tests, SARS Virus (drug effects), Tungsten Compounds (chemistry), Tungsten Compounds (pharmacology), Vero Cells, Virus Replication (drug effects).
- MESH :
- chemical , analysis : Hepatitis B Core Antigens, Hepatitis B Surface Antigens, Hepatitis B e Antigens.
- chemical , chemical synthesis : Antiviral Agents.
- chemical , chemistry : Antiviral Agents, Tungsten Compounds.
- chemical , drug effects : DNA, Viral.
- chemical , pharmacology : Antiviral Agents, Tungsten Compounds.
- drug effects : Cytopathogenic Effect, Viral, Hepatitis B virus, SARS Virus, Virus Replication.
- Animals, Chlorocebus aethiops, Hep G2 Cells, Humans, Microbial Sensitivity Tests, Vero Cells.
Abstract
A structural determined heteropolytungstate, [K(4)(H(2)O)(8)Cl][K(4)(H(2)O)(4)PTi(2)W(10)O(40)]·NH(2)OH 1, has been synthesized and evaluated for in vitro antiviral activities against hepatitis B (HBV) and SARS virus. The identity and high purity of compound 1 were confirmed by elemental analysis, NMR, IR analysis and single-crystal X-ray diffraction. The compound 1, evaluated in HepG 2.2.15 cells expressing permanently HBV, significantly reduced the levels of HBV antigens and HBV DNA in a dose-dependent and time-dependent manner. EC(50) values were determined to be 54μM for HBeAg, 61μM for HBsAg and 2.66μM for supernatant HBV DNA, as compared to 1671, 1570, 169μM, respectively, for the commercially-available hepatitis B drug adefovir dipivoxil (ADV). Intracellular cccDNA, pgRNA and HBcAg were also found to be decreased by compound 1 in a concentration-dependent manner. Cytotoxicity results showed that compound 1 has low toxicity in HepG 2 cells with CC(50) value of 515.20μM. The results indicate that compound 1 can efficiently inhibit HBV replication in HepG 2.2.15 cells line in vitro. Additionally, compound 1 also shows high anti-SARS activity at an EC(50) of 7.08μM and toxicity with a CC(50) of 118.6μM against MDCK cells.
DOI: 10.1016/j.antiviral.2011.11.003
PubMed: 22127069
Affiliations:
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last="Wang">Juan Wang</name></author><author><name sortKey="Jiang, Yanfang" sort="Jiang, Yanfang" uniqKey="Jiang Y" first="Yanfang" last="Jiang">Yanfang Jiang</name></author><author><name sortKey="Li, Jinhua" sort="Li, Jinhua" uniqKey="Li J" first="Jinhua" last="Li">Jinhua Li</name></author><author><name sortKey="Yuan, Ye" sort="Yuan, Ye" uniqKey="Yuan Y" first="Ye" last="Yuan">Ye Yuan</name></author><author><name sortKey="Zhang, Shiyao" sort="Zhang, Shiyao" uniqKey="Zhang S" first="Shiyao" last="Zhang">Shiyao Zhang</name></author><author><name sortKey="Xu, Kun" sort="Xu, Kun" uniqKey="Xu K" first="Kun" last="Xu">Kun Xu</name></author><author><name sortKey="Li, Yangguang" sort="Li, Yangguang" uniqKey="Li Y" first="Yangguang" last="Li">Yangguang Li</name></author><author><name sortKey="Li, Juan" sort="Li, Juan" uniqKey="Li J" first="Juan" last="Li">Juan Li</name></author><author><name sortKey="Niu, Junqi" sort="Niu, Junqi" uniqKey="Niu J" first="Junqi" last="Niu">Junqi 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H" first="Hong" last="Zhang">Hong Zhang</name></author><author><name sortKey="Wang, Juan" sort="Wang, Juan" uniqKey="Wang J" first="Juan" last="Wang">Juan Wang</name></author><author><name sortKey="Jiang, Yanfang" sort="Jiang, Yanfang" uniqKey="Jiang Y" first="Yanfang" last="Jiang">Yanfang Jiang</name></author><author><name sortKey="Li, Jinhua" sort="Li, Jinhua" uniqKey="Li J" first="Jinhua" last="Li">Jinhua Li</name></author><author><name sortKey="Yuan, Ye" sort="Yuan, Ye" uniqKey="Yuan Y" first="Ye" last="Yuan">Ye Yuan</name></author><author><name sortKey="Zhang, Shiyao" sort="Zhang, Shiyao" uniqKey="Zhang S" first="Shiyao" last="Zhang">Shiyao Zhang</name></author><author><name sortKey="Xu, Kun" sort="Xu, Kun" uniqKey="Xu K" first="Kun" last="Xu">Kun Xu</name></author><author><name sortKey="Li, Yangguang" sort="Li, Yangguang" uniqKey="Li Y" first="Yangguang" last="Li">Yangguang Li</name></author><author><name sortKey="Li, Juan" sort="Li, Juan" uniqKey="Li J" first="Juan" last="Li">Juan Li</name></author><author><name sortKey="Niu, Junqi" sort="Niu, Junqi" uniqKey="Niu J" first="Junqi" last="Niu">Junqi Niu</name></author><author><name sortKey="Wang, Enbo" sort="Wang, Enbo" uniqKey="Wang E" first="Enbo" last="Wang">Enbo Wang</name></author></analytic><series><title level="j">Antiviral research</title><idno type="eISSN">1872-9096</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antiviral Agents (chemical synthesis)</term><term>Antiviral Agents (chemistry)</term><term>Antiviral Agents (pharmacology)</term><term>Chlorocebus aethiops</term><term>Cytopathogenic Effect, Viral (drug effects)</term><term>DNA, Viral (drug effects)</term><term>Hep G2 Cells</term><term>Hepatitis B Core Antigens (analysis)</term><term>Hepatitis B Surface Antigens (analysis)</term><term>Hepatitis B e Antigens (analysis)</term><term>Hepatitis B virus (drug effects)</term><term>Humans</term><term>Microbial Sensitivity Tests</term><term>SARS Virus (drug effects)</term><term>Tungsten Compounds (chemistry)</term><term>Tungsten Compounds (pharmacology)</term><term>Vero Cells</term><term>Virus Replication (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral ()</term><term>Animaux</term><term>Antigènes de la nucléocapside du virus de l'hépatite virale B (analyse)</term><term>Antigènes de surface du virus de l'hépatite B (analyse)</term><term>Antigènes e du virus de l'hépatite virale B (analyse)</term><term>Antiviraux ()</term><term>Antiviraux (pharmacologie)</term><term>Antiviraux (synthèse chimique)</term><term>Cellules HepG2</term><term>Cellules Vero</term><term>Composés du tungstène ()</term><term>Composés du tungstène (pharmacologie)</term><term>Effet cytopathogène viral ()</term><term>Humains</term><term>Réplication virale ()</term><term>Tests de sensibilité microbienne</term><term>Virus de l'hépatite B ()</term><term>Virus du SRAS ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Hepatitis B Core Antigens</term><term>Hepatitis B Surface Antigens</term><term>Hepatitis B e Antigens</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>Antiviral Agents</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antiviral Agents</term><term>Tungsten Compounds</term></keywords><keywords scheme="MESH" type="chemical" qualifier="drug effects" xml:lang="en"><term>DNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term><term>Tungsten Compounds</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Antigènes de la nucléocapside du virus de l'hépatite virale B</term><term>Antigènes de surface du virus de l'hépatite B</term><term>Antigènes e du virus de l'hépatite virale B</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cytopathogenic Effect, Viral</term><term>Hepatitis B virus</term><term>SARS Virus</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antiviraux</term><term>Composés du tungstène</term></keywords><keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>Antiviraux</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Hep G2 Cells</term><term>Humans</term><term>Microbial Sensitivity Tests</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN viral</term><term>Animaux</term><term>Antiviraux</term><term>Cellules HepG2</term><term>Cellules Vero</term><term>Composés du tungstène</term><term>Effet cytopathogène viral</term><term>Humains</term><term>Réplication virale</term><term>Tests de sensibilité microbienne</term><term>Virus de l'hépatite B</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A structural determined heteropolytungstate, [K(4)(H(2)O)(8)Cl][K(4)(H(2)O)(4)PTi(2)W(10)O(40)]·NH(2)OH 1, has been synthesized and evaluated for in vitro antiviral activities against hepatitis B (HBV) and SARS virus. The identity and high purity of compound 1 were confirmed by elemental analysis, NMR, IR analysis and single-crystal X-ray diffraction. The compound 1, evaluated in HepG 2.2.15 cells expressing permanently HBV, significantly reduced the levels of HBV antigens and HBV DNA in a dose-dependent and time-dependent manner. EC(50) values were determined to be 54μM for HBeAg, 61μM for HBsAg and 2.66μM for supernatant HBV DNA, as compared to 1671, 1570, 169μM, respectively, for the commercially-available hepatitis B drug adefovir dipivoxil (ADV). Intracellular cccDNA, pgRNA and HBcAg were also found to be decreased by compound 1 in a concentration-dependent manner. Cytotoxicity results showed that compound 1 has low toxicity in HepG 2 cells with CC(50) value of 515.20μM. The results indicate that compound 1 can efficiently inhibit HBV replication in HepG 2.2.15 cells line in vitro. Additionally, compound 1 also shows high anti-SARS activity at an EC(50) of 7.08μM and toxicity with a CC(50) of 118.6μM against MDCK cells.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Jilin</li></region><settlement><li>Changchun</li></settlement><orgName><li>Université de Jilin</li></orgName></list><tree><noCountry><name sortKey="Jiang, Yanfang" sort="Jiang, Yanfang" uniqKey="Jiang Y" first="Yanfang" last="Jiang">Yanfang Jiang</name><name sortKey="Li, Jinhua" sort="Li, Jinhua" uniqKey="Li J" first="Jinhua" last="Li">Jinhua Li</name><name sortKey="Li, Juan" sort="Li, Juan" uniqKey="Li J" first="Juan" last="Li">Juan Li</name><name sortKey="Li, Yangguang" sort="Li, Yangguang" uniqKey="Li Y" first="Yangguang" last="Li">Yangguang Li</name><name sortKey="Niu, Junqi" sort="Niu, Junqi" uniqKey="Niu J" first="Junqi" last="Niu">Junqi Niu</name><name sortKey="Wang, Enbo" sort="Wang, Enbo" uniqKey="Wang E" first="Enbo" last="Wang">Enbo Wang</name><name sortKey="Wang, Juan" sort="Wang, Juan" uniqKey="Wang J" first="Juan" last="Wang">Juan Wang</name><name sortKey="Xu, Kun" sort="Xu, Kun" uniqKey="Xu K" first="Kun" last="Xu">Kun Xu</name><name sortKey="Yuan, Ye" sort="Yuan, Ye" uniqKey="Yuan Y" first="Ye" last="Yuan">Ye Yuan</name><name sortKey="Zhang, Hong" sort="Zhang, Hong" uniqKey="Zhang H" first="Hong" last="Zhang">Hong Zhang</name><name sortKey="Zhang, Shiyao" sort="Zhang, Shiyao" uniqKey="Zhang S" first="Shiyao" last="Zhang">Shiyao Zhang</name></noCountry><country name="République populaire de Chine"><region name="Jilin"><name sortKey="Qi, Yan Fei" sort="Qi, Yan Fei" uniqKey="Qi Y" first="Yan-Fei" last="Qi">Yan-Fei Qi</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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